A new technology for analyzing intact protein charge variants is urgently needed in cancer biomarker discovery because the charge variants of proteins are potential biomarkers for cancer. Prostate-specific antigen (PSA) is a FDA approved prostate cancer biomarker with many different charge variant glycoforms. The drawback of PSA is that different prostate diseases also increase the PSA level, leading to the false positives in cancer diagnosis. Isoelectric focusing (IEF) is a powerful tool of separating protein isoforms based on slight isoelectric point (PI) changes. Although capillary isoelectric focusing is faster in time and economic in sample usage, the pH gradient drift and electroosmotic flow cause problems in resolution and reproducibility. We describe the use of packed capillaries IEF show high resolution of PSA glycoforms separation with comparable speed and better reproducibility.

Isoelectric focusing of prostate-specific antigen in silica packed capillaries

248th National Meeting (2014)

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