Surface molecular imprinting is one of the most promising imprinting technique for protein molecules. In this work, a series of novel protein molecularly imprinted polymers (MIPs) were successfully prepared on the surface of multiwall carbon nanotubes (MCNTs) through a surface molecular imprinting technique by using acrylamide modified MCNTs as the substrates, bovine serum albumin (BSA) as the template molecules, allyl-functionalized ionic liquids as the functional monomers, N,N-methylenebisacrylamide (NNMBA) as the cross-linker, ammonium persulphate (APS) and N,N,N',N'-tetramethylethylenediamine (TEMED) as the initiating system. The surface morphology, specific surface area, adsorption-desorption-readsorption ability, and the selective recognition ability of the synthesized BSA MIPs were evaluated. Adsorption experiments showed that the amounts of BSA adsorbed by these four ionic liquid-based MIPs were much larger than the non-imprinted polymers. The MIPs synthesized using 1-allyl-3-imethylimidazolium hexafluorophosphate ([AMIM][PF6]) or 1-allyl-3-imethylimidazolium trifluoromethanesulfonate ([AMIM][CF3SO3]) ionic liquids as functional monomers had higher adsorption capacity and better imprinting effect to BSA compared with the traditional acrylamide-based MIPs. For the developed [AMIM][PF6] and [AMIM][CF3SO3]-based MIPs, the maximum adsorption capacities were 52.84 and 66.73 mg g-1, ant the imprinting factors were 3.36 and 6.13, respectively. These ionic liquids-based MIPs also had better selective recognition ability for BSA than for such molecules as human serum albumin, lysozyme, trypsin and bovine hemoglobin (BHb). As concluded from the competitive adsorption experiment between BSA and BHb, the selective factor of the [AMIM][PF6] and [AMIM][CF3SO3]-based MIPs for BSA were 7.15 and 10.05, respectively.The results obtained from this work indicated that ionic liquids would be ideal functional monomers for the development of protein molecularly imprinted polymers.

Preparation and characterization of ionic liquid-based bovine serum albumin molecularly imprinted polymers on the surface of multiwall carbon nanotubes

248th National Meeting (2014)

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