Biobeds are cheap and environmental friendly solutions to remediate impacted points. Composed by a mixture of soil, peat and nutrients gathered from agricultural wastes, biobeds foster the growth of microorganisms that metabolize the pesticides spilled after washing containers or spoiled from application equipments. This work present a general strategy to built up biobeds based in the biotransformation capability of basidiomycetes and the development of validated analytical methodology to assess the remediation process along the biobed development. A screening of native basidiomycetes yielded two strains able to grow using endosulfan and chlorpyrifos as sole carbon source, degrading them up to 95% in YNB medium. Cereals brans were chosen as they support fungal growth keeping their biotransformation ability. Finally, a biobed that can metabolize both pesticides was optimized after evaluating their transformation products, different soil compositions, the native microbiota and the aeration to ascertain the usefulness of these environmentally friendly tool.

Bioremediation of recalcitrant pesticides using biobeds

248th National Meeting (2014)

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