The United States is the largest soybean (Glycine max) producer (35% of the total production) in the world.  The majority of the soybean crop is processed into vegetable oil and soybean meal.  Soybean is considered a major human dietary source of protein and essential amino acids.  However soybean and its products can produce allergenic responses in sensitized individuals.  HPS (soybean hydrophobic protein) and Gly m 4 (pathogenesis related protein PR-10) are two of several soybean proteins which have been identified to cause allergenic reactions in humans.  In 2010, 93% of soybeans cultivated for the commercial market in the United States were genetically modified.  World-wide, regulatory agencies have been focusing on the effects of these transgenes on the expression levels of known allergens.  However, current compositional analysis of genetically modified (GM) crops (over 20 years of data) has shown that introduction of transgenes does not adversely affect the composition of GM crops.  Moreover, crop composition is highly influenced by environmental and endogenous genotypic factors.  To test this hypothesis (environmental and endogenous genotypic factors affect the levels of expressed allergens), we analyzed 160 soybean samples from 6 varieties, grown during the 2012 season at 10 different field locations throughout the major US growing regions.  A validated two-dimensional liquid chromatography method with ultraviolet and mass spectrometric detection (2DLC�UV/MS) was applied to quantify the HPS and Gly m 4 expression level in the 160 soybean samples. Results from the analysis of the soybean samples will be discussed.

Biovariability of two endogenous soybean allergens, HPS and Gly m 4

248th National Meeting (2014)

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