Fluorogenic response, i.e. either a change in fluorescent quantum yield or a change in emissive wave length of probes, is a highly desirable trait in fluorescence microscopy. Such response markedly improves the signal to noise ratio in a given experiment and serves as an excellent choice in biological investigations where unbound probe washing is a problem. Among the reactions utilized to bring fluorogenic response, the �click� reaction is most widely used. The reaction needs two coupling partners, the azide and alkyne, and is highly selective, fast, forming 1,2,3-triazole. By utilizing the electronic changes associated with the triazole formation, the reaction is skillfully used in imparting fluorogenity to various fluorophore scaffolds. However, these scaffolds are limited in number and efforts are needed not only to develop new fluorophore scaffolds but also to improve their associated properties. Towards these goals we bring forth a novel scaffold, Naphthalic Ynimide (naphthalic dicarboximide having N-alkyne functionality). The current study discusses their synthesis, fluorescent properties, both before and after the �click� reaction, and the reaction kinetics with their azide counter part.

Naphthalic ynimides as novel fluorogenic �Click� probes

248th National Meeting (2014)

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