Poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA) is a dually reactive block copolymer that can be used to tailor surface properties. Upon PGMA-b-PVDMA surface coating, GMA blocks react with oxide-bearing surfaces, while VDMA blocks can be used for secondary modification with nucleophiles. In this communication, we use photolithography to create three-dimensional surface patterns of PGMA-b-PVDMA and then further modify these surfaces with lectin proteins for the selective capture of bacterial cells from solution. The high concentrations of VDMA groups present within these polymeric structures allow for the coupling of large amounts of lectin. We show that the combination of high lectin concentration, high surface roughness, and micron-scale surface topology characteristic of the PGMA-b-PVDMA structures create a high-avidity surface for efficient capture of bacterial cells. We further show that varying the pattern shape and density allows for control over the level of aggregation within the captured bacteria population (Figure 1). These functional polymer surfaces may advance the capabilities of affinity-based bacterial isolation by providing high capture efficiency while also preventing extensive biofouling.Figure 1. (Top) 100x brightfield-fluorescent images of P. fluorescens microbes captured onto lectin-functionalized surfaces containing linear PGMA-b-PVDMA microstructures (scale bar = 10 �m) and (bottom) corresponding histogram plots of aggregate size distribution measured over a 2.0 mm2 area at a pattern pitch of (A) 25 �m, (B) 10 �m and (C) 7 �m.

Bioactive block copolymer surfaces for control of microbe capture and aggregation

248th National Meeting (2014)

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