A new type of nano-fibrous polymeric system is described, in which chemical modifications can be easily implemented photochemically to introduce new functionalities. We have introduced peptides and proteins onto the surface of the fibers, for use as cell seeding scaffolds. Our process begins by using a co-extrusion and multiplication method. Poly(e-caprolactone) (PCL) and polyethylene oxide (PEO) are co-extruded through a series of dye multipliers within the extrusion line to yield a multi-component polymeric tape. The resulting tape is comprised of a matrix of PCL nanofibers embedded within a PEO filler. The PEO layer is removed with a simple water wash to yield PCL nanofibers of controlled cross-sectional dimensions. Once the fibrous mat is processed, the fibers can be photochemically modified with functionalized benzophenones to yield new functional groups on the fiber surface. We have incorporated reactive handles for performing the copper catalyzed azide-alkyne cycloaddition (CuAAC) reaction or oxime ligation, for example. On the surface of the PCL fibers, we have attached numerous substrates, such as small molecules, peptides, and proteins. Subsequent cell-based studies have shown that, following immobilization, peptides are available to provide biochemical cues to promote cellular phenomena. Several peptides and peptide gradients have been attached to the fibers and were able to increase cellular adhesion and spreading versus PCL control surfaces. The co-extruded fibers are of clear use due to their modularity and scalability, opening a potentially new avenue for biomaterials fabrication.

Surface modification of continuously processed polymer nanofibers: Incorporation of peptides, proteins, and small molecules

248th National Meeting (2014)

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