The redox potentials of aged hemoglobins were examined to investigate the potential effect of prolonged storage on the nitrite reductase activity of red blood cells. Young Hb, old Hb, total Hb, and centrifuged Hb yielded redox potentials of -75.2 mV, -89.8 mV, -84.0 mV, and -73.2 mV respectively (Ag/AgCl reference potential). The effect of age on the nitrite reductase activity of erythrocytes under 1% O2 and 21% O2 was examined as well, with old erythrocytes causing the leasat activity and total erythrocytes causing the most. The trend in nitrite reductase activity for erythrocytes did not match the trend in reduction potential for hemoglobin.

Effect of age on redox potential and nitrite reductase activity of hemoglobin

248th National Meeting (2014)

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