Cynanchum komarovii is a desert plant that has been used as analgesic, anthelminthic, and antidiarrheal, but also as an herbal medicine to treat cholecystitis in people. We have found that the protein extractions from C. komarovii seeds have strong antifungal activity. There is strong interest to develop protein medication and antifungal pesticides from C. komaroviifor pharmacological or other uses. In this study, a novel plant annexin, CkANN, was isolated from C. komarovii. Isolation of antifungal proteins from C. komarovii seeds will be explained. The protein showed antifungal activity against fungal growth of Fusarium oxysporum. The full-length cDNA was cloned by RT-PCR and RACE-PCR according to the partial protein sequences obtained by nanoESI-MS/MS. The CkANN expression patterns of C. komarovii displayed more abundant mRNA in leaf, flower, and root than stem. The transcription level of CkANN had a significant increase under the stress of ABA, SA, MeJA, NaCl, which indicates that CkANN may play an important role in response to abiotic stresses. CkANN was constructed into pCAMBIA1304 expression vector, were transformed into cotton(CCRI 45). The CkANN protein was located in the cell membrane by CkANN::GFP fusion protein in transgenic Arabidopsis. Furthermore, over-expression of CkANN significantly enhanced the resistance of Cotton against F. oxysporum. Data demonstrating resistance of transgenic cotton to F. oxysporum compared to WT plants will be shown. These results implicate the CkANN protein is capable of protecting plants against fungal diseases. [This work was supported by the Program of National Nature Science Foundation of China (grant no. 31071751) and the Genetically Modified Organism Breeding Major Project (grant no. 2013ZX08005 - 002)]

Purification and characterization of an antifungal protein from <i>Cynanchun komarovii</i> seeds confers <i>Fusarium oxysporum</i> resistance in cotton

248th National Meeting (2014)

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Purification and characterization of an antifungal protein from Cynanchun komarovii seeds confers Fusarium oxysporum resistance in cotton