Chesulfamide (N - (2- trifluoromethyl - 4 -chlorphenyl) - 2 - oxocyclohexyl sulfonamide) is a novel fungicide, which had fungicidal activity against Botrytis cinerea with the EC50 values of 2.12 �g mL-1. In order to elucidate the biochemical mode of action of chesulfamide against B. cinerea, effects of chesulfamide on ultrastructural of hyphae, electrolyte leakage and respiration of mycelia suspension, including the content changes of biomacromolecules (DNA, protein, polysaccharide and lipids) caused by chesulfamide, were studied. The results showed that chesulfamide induced irregular swelling of hyphae, vacuolation of cytoplasm and extensive thickness of cell wall. Chesulfamide could enhance the penetrability of cellular membrane and the content of the water-soluble proteins in mycelia, and reduced the content of the DNA, polysaccharide in mycelia. Therefore, we preliminary presumed that chesulfamide exhibited multiple modes of action including the effect on cell wall or cell membrane biosynthesis or the effect on DNA directly or indirectly.

Primary mode of action of novel fungicide chesulfamide against Botrytis cinerea

248th National Meeting (2014)

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