The peanut plant produces stilbenoids as a defense mechanism against biotic and abiotic stress. Among these polyphenolic compounds are resveratrol and its prenylated analogs arachidin-1 and arachidin-3.  We developed hairy root cultures from several cultivars of peanut and demonstrated that these biological systems can be induced to produce resveratrol and arachidins upon treatment with elicitors. To increase the yield of the arachidins, cyclodextrin and metabolic precursors were added in combination with the elicitor methyl jasmonate. More than 90% of the arachidins secreted into the culture medium upon elicitation. High performance counter current chromatography was used to purify the arachidins from the culture medium. Fractions of arachidin-1 and arachidin-3 with over 95% purity were obtained. The arachidins show increased antioxidant properties when compared to resveratrol. These compounds also exhibit increased lipophilicity and potentially better cellular uptake due to the prenylated side chain in their structures.  This presentation will discuss the different bioactivities that have been associated with the arachidins and their potential use as functional ingredients.

Bioproduction and biological activities of arachidin-1 and arachidin-3: Potential functional ingredients derived from peanut

248th National Meeting (2014)

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