Enantiomeric separation of chiral NSAID drugs and their metabolites is essential in order to evaluate the implication of stereoselectivity both in terms of drug action and absorption. A rapid and stereoselective high-performance liquid chromatography method based on a (2-hydroxypropyl)-�-cyclodextrin chiral mobile phase additive (CMPA) was studied. A separation of dexibuprofen tablets and racemic ibuprofen tablets was carried out using Thermo Scientific Hypercarb (150mm x 4.6 mm, 5 �m) column, with a UV detector set at 254 nm, mobile phase consisting of acetonitrile, 25 mM HP-�-cyclodextrin, and 0.1 M sulfuric acid (40:40:20 v/v/v, pH: 2.5). Further research is required on the effect of altering column type, mobile phase composition, and pH on improving resolution and efficiency of chiral methodology.

Novel enantioselective chiral separation of racemic ibuprofen using reversed-phase high-performance liquid chromatography

248th National Meeting (2014)

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