NK130102, which show high activity against lepidoptera insect pests, is a novel insecticide developed in China. The LC50 and LC95 values of NK130102 against Ostrinia furnacalis were 0.2 and 8.8 mg g-1 respectively. The PGM transcriptome sequencing was used to evaluate changes in the global gene expression of O. furnacalis treated with NK130102 at the dose of 8.8 mg g-1 for 48h. A total of 35430 unigenes were obtained from the transcriptome analysis, and 3725 unigenes uniquely existed in NK130102 treated O. furnacalis. By comparing the expression of genes in O. furnacalis treated and untreated with NK130102, we identified 506 and 10 unigenes showing more than 5-fold increase and decrease, respectively, in expression upon NK130102 treatment. The transcriptome sequences were annotated with nonredundant (Nr) protein database, gene ontology (GO), cluster of orthologous groups of proteins (COG), and KEGG orthology (KO). The enzymes (P450 and GST) potentially involved in insecticide metabolism and those encoding the targets of the major chemical classes of insecticides were manually curated and annotated. The ryanodine receptor (RyR) gene was up-regulated 3.7 fold by NK130102, and the muscle M-line assembly protein involved in vascular smooth muscle contraction in calcium signaling pathway was up-regulated 26.9 fold in O. furnacalis treated with NK130102. The O. furnacalis transcriptome data provide gene expression data that would provide useful information in understanding the mechanisms for the responses of lepidoptera insect pests to novel insecticide NK130102. In particular, the findings of this investigation will facilitate identification of genes involved in insecticide resistance and the design of new compounds for control of O. furnacalis.

Gene transcription profiling of <i>Ostrinia furnacalis</i> treated with the novel insecticide NK130102

248th National Meeting (2014)

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Gene transcription profiling of Ostrinia furnacalis treated with the novel insecticide NK130102