Spinetoram is a broad-spectrum chewing insecticide. It is a semi-synthetic product composed primarily of two major factors, J and L. To support method development, metabolism, and environmental fate studies on spinetoram, there was a need to prepare isotopically labeled standards of both spinetoram J and L. Stable isotopes were prepared by incorporation of deuterium and carbon-13. The macrolide ring was labeled with carbon-14 via fermentation of spinosyn factors J and L by an external vendor using carbon-14 labeled acetate. The macrolide labeled spinosyn factors J and L were then carried on to spinetoram J and L via chemical synthesis. In addition, metabolites were prepared with the macrolide label along with analogs of both spinetoram J and L that contained stable isotopes in addition to carbon-14. The synthesis of spinetoram J and L, and their major metabolites, containing stable and radioactive isotopes will be discussed.

Preparation of isotopically-labeled standards of spinetoram

248th National Meeting (2014)

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