Rice (Oryza sativa) is one of the most important cereal crops and provides the bulk of daily calories for humans. Recently, much attention has been paid to bioactive components in rice. In the present study, phenolic components and oligosaccharides from purple rice (cv. Asamurasaki) bran treated with cellulase (Cellulosin T2) were investigated to develop new uses related to human health. Cellulosin was used for hydrolyzing plant cell walls to produce oligosaccharides and phenolic components. The amounts extracted by water or 85% methanol were increased by about a factor of two in wt %.  Their compositions were analyzed by HPLC and spectroscopic methods including NMR.We studied the intestinal permeability of these compounds using the Caco-2 permeability assay. Studying the permeability of compounds across a Caco-2 cell monolayer is an established in vitro method to screen for oral absorption and to evaluate the mechanism of transport. HPLC analysis of samples derived from Caco-2 cell studies shows that several components transport across the Caco-2 cell monolayer.The antioxidant power in component transport across the Caco-2 cell monolayer was determined using the 'PAO' test. This assay evaluates Cu+ levels derived by reduction of Cu2+ from the action of antioxidant present in the sample. The stable complex between Cu+ and bathocuproine occurs at 490 nm. The PAO test showed that the component transport across the Caco-2 cell monolayer shows the same level of antioxidant activity as the apical test sample. Our studies have shown that dietary supplementation with rice powder rich in anthocyanins and oligosaccharides could be effective in reducing oxidative stress. But the structure of the components showing anti-oxidant effects should be the subject of further investigation.

Antioxidant activity and Caco-2 cell permeability of compounds from black rice bran treated with cellulase (Cellulosin T2)

248th National Meeting (2014)

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