Oil extraction of Jatropha curcas seed were created the large amount of the cake by-product. Deoiled J. curcas seed cake is rich of protein and other different nutrients like minerals, and amino acids were suitable to use as the nutrients for microbial growth. Deoiled J. curcas seed cake was assessed as substrate for the production of extracellular xylanase by Bacillus smithii G16 in solid state fermentation. The seed cake was efficiently utilized by B. smithii G16 for its growth and phorbol ester degradation during which it produced extracellular xylanase. Initial screening process identified the best xylanase production strains, and then different parameters such as incubation time (day 1-9), initial moisture content (40%, 50%, 60%), incubation temperature (25, 35, 50oC), inoculum level (106, 107, 108 CFU/g), carbon sources (1% glucose, 1% maltose, 1% starch), and nitrogen sources (0.5% NaNO3, 0.5% NH4NO3, 0.5% peptone) of the medium were optimized. The optimum conditions for the xylanase production by B. smithii G16 obtained from this experiment were 50% substrate moisture, 35oC incubation temperature, a growth period of 48 h, inoculated with 108 CFU per 1 g cake, supplemented with 1% glucose and 0.5% peptone, 26.55 � 0.37 U xylanase activity per gram of Jatropha seed cake was obtained. The xylanase was useful in bleach craft pulp, to increase the brightness in paper industry, to improve the digestibility of animal feed and to clarify the juices in the food industry. Solid-state fermentation of deoiled J. curcascake appears a potentially viable approach for its effective utilization.

Optimization of solid state fermentation conditions for the production of xylanase by <i>Bacillus smithii</i> using deoiled <i>Jatropha curcas</i> seed cake

248th National Meeting (2014)

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Optimization of solid state fermentation conditions for the production of xylanase by Bacillus smithii using deoiled Jatropha curcas seed cake