An area of great interest to the bioanalysis and biomedical communities is that concerning the ability to detect, image, and differentiate diseased cells by use of upregulated markers that trigger molecular probe reporting.  At present, a topic of intense investigation concerns �cloaked� probes that are fluorescently silent due to quenching events associated with covalently attached functional groups, but whose fluorescent signature is revealed upon upregulated marker-triggered removal of the quenching-associated functional group.  This presentation addresses a new class of cloaked probes whose fluorescent reporter form is produced upon probe activation by a redox-enzyme that is intimately linked to a large number of cancerous tissues.  Passive targeting of cancer cells by these turn-on probes results in rapid observation of unprecedented positive-to-negative values in one- and two-photon microscopy studies, as well as unaided eye visualization of low numbers of cancer cells.

Highly selective cloaked fluorogenic probes for detection and imaging of disease markers

248th National Meeting (2014)

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