Chlorantraniliprole (anthranilic diamides) and flubendiamide (phthalic diamides) as novel diamide insecticides for the control of Lepidopteran and Coleopteran pests with the same mode of action were introduced into the Asian market. They activate insect ryanodine (Ry receptors, RyR) and cause muscle contraction, paralysis and death. The present study explored the different effects on the L-type Ca2+ channel and the calcium channels in the endoplasmic reticulumn (ER) in the central neurons isolated from the third instar larvae of Spodoptera exigua using whole-cell patch clamp, calcium imaging and Non-invasive Micro-test Technology (NMT). NMT recordings reveal that the extracellular calcium concentration was reduced after the neurons were treated with chlorantraniliprole. Calaium imaging technique shows that after the brief application of flubendiamide, re-introduction of standard saline allowed depleted calcium stores to become refilled and then continued to stimulate a transient elevation in [Ca2+]i by chlorantraniliprole in the absence of external calcium and the converse processing does not bring the same results. The experiments showed that the possible calcium signaling pathway of chlorantraniliprole might be different from flubendiamide.

Modulation of calcium channels in the central neurons of <i>Spodoptera exigua</i> by chlorantraniliprole and flubendiamide

248th National Meeting (2014)

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Modulation of calcium channels in the central neurons of Spodoptera exigua by chlorantraniliprole and flubendiamide