The nAChR is the target site of neonicotinoid insecticides. The neonicotinoid resistance of cotton aphid, Aphis gossypii Glover, was reported first in Miyazaki and Oita prefs. in Japan. The resistance level was different between chemical structures of neonicotinoids. In both strains, the resistance level to the compounds containing cyano substitute (e.g. acetamiprid) was lower than those of nitro (e.g. imidacloprid). We confirmed a single point mutation (R81T) in the b subunit of the nAChR, most likely main factor of the resistance mechanism. In addition, electrophysiological study, TEVC method, showed that the potency of imidacloprid (nitro type) against the resistant nAchR was decreased, but not acetamiprid (cyano type). The difference of the interactions to the resistant nAchR between nitro and cyano substitutes might confer the susceptibilities to the 2 neonicotinoids in the resistant aphids.

R81T mutation in the nicotinic acetylcholine receptor(nAChR) is associated with differential resistance level to acetamiprid and imidacloprid in the <i>Aphis gossypii</i>

248th National Meeting (2014)

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R81T mutation in the nicotinic acetylcholine receptor(nAChR) is associated with differential resistance level to acetamiprid and imidacloprid in the Aphis gossypii