Advancement of RNAi therapies is mainly hindered by the development of efficient delivery vehicles. The ability to create small size (< 30 nm) oligonucleotide nanoparticles is essential for many aspects of the delivery process but is often overlooked. In this report, we describe di-block star polymers that can reproducibly complex double-stranded oligonucleotides into monodisperse nanoparticles with 15, 23 or 30 nm in diameter. The polymer-nucleic acid nanoparticles have a core-shell architecture with dense PEG brush coating. We characterized these nanoparticles using ITC, DLS, FRET, FCS, TIRF and TEM. In addition to small size, these nanoparticles have neutral zeta potentials making the presented polymer architecture a very attractive platform for investigation of yet poorly studied polyplex size range for siRNA and antisense oligonucleotide delivery applications.

Precisely tunable engineering of sub-30 nm monodisperse oligonucleotide nanoparticles

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.