The chemical composition of phospholipids in lecithin strongly influences the physicochemical properties and performance of different type of lecithin. Consequently, a rapid and reliable characterization of phospholipid composition is crucial for many applications. However, due to the complex nature and poor solubility of these phospholipids in water, it is not practical to use the most common analytical approach of reverse-phase HPLC/MS/MS. Consequently, there are not many technical publications describing phospholipid composition. In the present study, an HILIC-electrospray ionization (ESI)-MS-MS method was developed for the separation and identification of the chemical composition of different phospholipid classes and molecular species.  A full separation of the entire composition was achieved in 20min. The further data process by using SIEVE software provided a full characterization of key chemicals in variety of lecithin products. HILIC/MS/MS was shown to be a useful tool for establishing the compositional profile of phospholipids in lecithin products.

Method development for rapid characterization of phospholipid composition in different lecithin products by HILIC/MS/MS

248th National Meeting (2014)

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