Acute lymphoblastic leukemia (ALL) is a very common cancer. It is characterized by an abnormal increase of immature white blood cells called 'blasts�, affecting the blood, bone marrow, and lymphoid system, which are all known as hematological neoplasms. An increase of reactive species production occurs in ALL with involvement of oxidative stress (OS) during all steps of disease. Reactive oxygen species particularly free radical (FR) induce tissue damage by lipid peroxidation. However, it is not always easy to measure this OS, requiring sensitive, selective, reliable and trusted methods. Malondialdehyde (MDA) is one of the lipid peroxidative products widely used as biomarker of cellular damage in plasmatic samples due to OS. So, monitor its contents can help in treating this disease. The objective of this study was to evaluate OS variations in 27 patients diagnosed with ALL compared to 18 healthy patients (control group), determined in plasma samples by High Performance Liquid Chromatography(HPLC), verifying feasibility of using MDA as biomarker. The extraction method uses TBA as chromogenic detected by HPLC with photodiode array validated method adapted from Bastos et al. (2012). The method showed to be linear (R�=0.9928) with 0.20 �mol L-1 for limit of detection and 0.66 �mol L-1 for quantification, repeatability (RSD 3.0%) with recovery rate within 103.0-109.0%. Plasma samples indicated substantially high OS for ALL patients compared to the healthy patients. Mean levels of MDA in the control group was of 0.91�0.18 mmol L-1 ranged from 0.58-1.20 mmol L-1, while for patients diagnosed with ALL the MDA level was of 2.17�0.77 mmol L-1 ranged from 0.97-3.67 mmol L-1. MDA levels analyzed by proposed method proved to be an adequate biomarker, since the groups showed significant differences (Unequal, p = 0.05) and division when groups performed the hierarchical clustering, representing an alternative to assist ALL patients under treatment.

Oxidative stress: MDA as biomarker in acute lymphoblastic leukemia

248th National Meeting (2014)

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