Polydopamine (PDA) thin films were fabricated from dopamine solutions either by a single-step polymerization-deposition method or by a controlled electrodeposition process and then employed in the construction of DNA-functionalized microelectrode arrays, nanoring arrays and magnetic nanoparticles for biosensing applications. While the bulk self-polymerization of dopamine monomers occurred in basic aqueous dopamine solutions (pH 8.5), polydopamine monolayers could be electrodeposited and patterned on the micron lengthscale from slightly acidic solutions (pH 6.5) by an electrochemically induced interfacial pH change. PDA multilayers with thicknesses varying from 1 to 5 nm were deposited and characterized from with a combination of electrochemistry, scanning angle surface plasmon resonance (SPR) and atomic force microscopy. DNA functionalization was accomplished by the reaction of amine-terminated single-stranded DNA (ssDNA) oligonucleotides with PDA-modified gold thin film microarray elements; these microarrays were subsequently employed in SPR imaging measurements of (i) DNA-DNA hybridization adsorption, (ii) DNA-protein bioaffinity adsorption and (iii) the hybridization adsorption of DNA-modified gold nanoparticles onto DNA-functionalized PDA nanorings.1. Fabrication of DNA Microarrays on Polydopamine-Modified Gold Thin Films for SPR Imaging Measurements Jennifer B. Wood, Megan W. Szyndler, Aaron R. Halpern, Kyunghee Cho, and Robert M. CornLangmuir 2013  29(34), 10868-108732. Electrodeposition of Polydopamine Thin Films for DNA Patterning and Microarrays Gabriel Loget, Jennifer B. Wood, Kyunghee Cho, Aaron R. Halpern, and Robert M. CornAnalytical Chemistry 2013  85(21), 9991-9995

Polydopamine thin film polymerization and electrodeposition for the fabrication of DNA-functionalized microarrays, nanoparticles, and nanorings

248th National Meeting (2014)

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