The up-regulation of P450 genes, resulting in increased levels of P450 proteins and P450 activities, has been widely recognized to associate with enhanced metabolic detoxification of insecticides and has been implicated in the development of insecticide resistance in insects. The different patterns of P450 gene expression in metabolic detoxification of insecticide-resistant insects have been found to be regulated by trans and/or cisfactors. However, no regulatory factors in insecticide resistance have yet been identified, and there has been no examination of the regulatory interaction of resistance genes. We examined P450 gene expression in the genome of mosquitoes Culex quinquefasciatus, characterized the functions of overexpressed P450 genes in insecticide resistance, and tested the effect of GPCRs in regulating resistant P450 gene overexpression. We, for the first time, provide novel information of a GPCR regulatory signaling pathway governing P450 gene expression and P450-mediated resistance in the Culex mosquitoes.

Cytochrome P450s, their expression, regulation, and role in insecticide resistance

248th National Meeting (2014)

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