We studied the interactions between perylene derivative Tel03 and different kinds of DNA sequences, such as telomerase primers and telomerase repeated sequences. Although Tel03 ligand could bind to both telomerase primers (P2) and telomerase repeated sequences (P1, P2, P3), the affinity was not the same. Tel03 has much stronger binding affinity to telomerase repeated sequences which could fold to G-quadruplex than telomerase primers (Fig.1). Based on the understanding above, we have utilized the property that graphene oxide (GO) possesses high fluorescence quenching ability toward ssDNA to design a novel fluorescent sensor for sensitive and specific telomerase activity detection.    Fig.1 Fluorescence of the telomerase primers and telomerase repeated sequences labeled with FAM in 200 nmol�L-1 Tel03 and 50 nmol�L-1GO mix solution. Fig.2 Fluorescence emission of telomerase primers incubated with various concentrations of cell extractsThe telomerase primer DNA was incubated with cell extract, strong emission was observed because repeated sequences extended by telomerase has turned to G-quaruplex structure, which could combine with Tel03 and break away from the surface of GO, leading to a high signal-to-background ratio (Fig.2). 50 cells could be detected in this method, and the regulation of DNA conformation points out a new way in the field of telomerase activity detection.This work is supported by Ministry of Science and Technology of China (Grant No. 2007CB936201, 2012CB720601)References Lu, C.H.; Yang, H.H.; Zhu, C. L.; Chen, X. G.; Chen, N. Angew. Chem. 2009  , 121, 4879-4881.Kim, N.W.; Platyszek, M.A.; Prowse, K.R.; et al. Science 1994  , 266, 2011-2015.He, S. J.; Song, B.; Li, D.; Zhu, C.; Qi, W.; Wen, Y.; Wang, L.; Song, S.; Fang, H.; Fan, C. Adv. Funct. Mater. 2010  , 20, 453-459.Liu, X.; Aizen, R.; Freeman, R.; Yehezkeli, O.; Willner, I. ACS Nano, 2012  , 6, 3553-3563.

Perylene derivative Tel03 selectively recognize human telomeric G-quadruplexDNA with Graphene oxide nanoprobe for sensitive fluorescent telomerase activity detection

248th National Meeting (2014)

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