Thiols play a significant role in defense against reactive oxygen species, detoxification, signal transduction, apoptosis, and various other cellular functions. A change in thiol level has been linked to the pathogenesis of a variety of disease states. Assessing thiol levels in live cells through fluorescence microscopy provides a way of direct visualization of thiol-related biochemical processes. Limited fluorescent agents are available to assess the level of thiols in live cells. We previously reported bis(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)sulfane (BNOS) that effectively imaged and quantified total thiols through fluorescence microscopy. In this poster, we would like to present a novel benzofurazan sulfide triphenylphosphonium fluorogenic compound that can also effectively image and quantify thiols in live cells through fluorescence microscopy and be complementary to BNOS with better sensitivity and water solubility. The synthesis, thiol specificity determination, fluorescence property characterization and total thiol imaging in live cells through fluorescence microscopy will be presented.

Live cell thiol imaging and quantification with a novel benzofurazan sulfide triphenylphosphonium fluorogenic compound

248th National Meeting (2014)

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