The promise of biosurfactants has a long and storied history of having commercial potential.  One of the major classes of biosurfactants is glycolipids that have the potential to replace a number of petroleum based surfactants due their sulfate and phosphate free nature while having superior surfactant properties.  This class of glycolipids are characterized by either a mono or di rhamnose head group and a 3-(hydroxyalkanoyloxy)alkanoic acid tail that varies in molecular weight according to a number of factors including feedstock, fermentation time and Pseudomonas strain.  Logos Technologies has developed three strains of Pseudomonads that produce rhamnolipids at high titers.In this presentation, Logos Technologies will discuss the three strains of Pseudomonads that have been isolated for the overproduction of both mono- and di-rhamnolipids.  Each strain has a unique rhamnolipid molecular weight profile for a specific feedstock. Results will be presented discussing how these two variables interact.  In addition, the rhamnolipids need to be isolated from the fermentation broth for utilization.  Data demonstrating a correlation between the molecular weight distribution of the rhamnolipid isolates and the CMC will be presented.  These biosurfactants have the characteristics and can meet the cost targets necessary for large scale commercialization.

Production of rhamnolipids from unique pseudomonads

248th National Meeting (2014)

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