Transfection of nucleic acid in cells is a critical task for a variety of biological experiments. However, a protocol that requires no optimization, and consists of a simple single step transformation for transfection is lacking. We have reformulated the Xfect� transfection reagent to prepare a single tube-single use format. This new format greatly simplifies the transfection protocol by allowing use of 1 to 10 ug of DNA with the same amount of transfection reagent which results in very high transfection efficiency in a variety of cell types.  We applied this high-efficiency transfection reagent to achieve single-step production of exosome-like nanoparticles in culture for the direct delivery of protein into mammalian cells. In addition, using this simplified protocol combined with an optimized lentiviral vector packaging mix resulted in a more robust and streamlined production of high-titer lentiviral particles for high efficiency gene delivery.

Reformulated transfection reagent with a single step protocol demonstrates high transfection efficiency with low cytotoxicity

248th National Meeting (2014)

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