The limit of detection for in vitro diagnostic devices needs to be decreased in order to be used for early disease detection. Here, we present a method that improves the enzyme-linked immunosorbent assay (ELISA). The commercially available streptavidin and synthetic biotinylated bovine serum albumin are added in a unique way to the antibody-antigen recognition event. Amplified detection signals are generated by the horseradish peroxidase conjugated streptavidin when combined with a commercially available reagent. The results enjoy a low detection limit in comparison to the standard sandwich ELISA method.

Protein networks for signal amplification

248th National Meeting (2014)

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