In this work, we report the responsive behaviour of hydrogels and their interfaces derived from biomimetic protein based polymers. Of particular interest to us are the protein-based elastomers, which are biocompatible and vastly superior to synthetic elastomers. Such elastomeric hydrogels[1] have gained increased research interest in biomedical applications for their remarkable self-assembly behaviour, tunable 3D porous structure, high resilience (elasticity), superior fatigue lifetime (durability), high water uptake, excellent biocompatibility and biological activity. Amongst them, intrinsically disordered proteins, such as elastin, resilin, and flagelliform spider silk have gained much interest in recent years due to their remarkable mechanical properties, particularly their outstanding elasticity and responsiveness. These proteins/polypeptides can be derived naturally (animal or insect sources) or by recombinant (bacterial expression) routes and can be crosslinked via physical or chemical approaches to obtain elastomeric hydrogels. The emergence of genetic engineering of synthetic polypeptides has also recently enabled to synthesize protein-based polymers artificially. These biomimetic elastomers have been of significant interest to understand the molecular basis of their elasticity. They could be used as 3D gels, nanoparticles, electrospun fibres, arrays, and injectable gels. The biological properties of these biomaterials can be tuned by proteins engineering or by fusion with other protein and bioactive domains such as cell binding domains to control cell response for wide range of tissue engineering applications e.g. muscles, cardiovascular tissues, and cartilage. Our recent studies [1-7] have demonstrated that real-time control over and switch-ability of biomolecule�surface interactions at interfaces play important role for a range of applications such as biosensing, drug delivery and tissue engineering, In this talk we discuss elastomeric gels/hydrogels that are responsive to stimuli such as light, temperature, pH and ionic strength and have outstanding elasticity and could be used to control protein adsorption/desorption and cell attachment/detachment for the development of next-generation smart bio-interfaces.References: 1. N. K. Dutta, M. Y. Truong, S. Mayavan, N. R. Choudhury, C. M. Elvin, M. Kim, R. Knott, K. M. Nairn and A. J. Hill, Angewand.Chem.-Int. Ed.  50: 4428-4431 (2011).2. N. K. Dutta, N. R. Choudhury, M. Y. Truong, M. Kim, C. M. Elvin and A. J. Hill, Biomaterials  30:4868-4876 (2009).3. M. Y. Truong, N. K. Dutta, N. R. Choudhury, M. Kim, C. M. Elvin, K. M. Nairn and A. J. Hill, Biomaterials  32: 8462-8473 (2011).4. M. Y. Truong, N. K. Dutta, N. R. Choudhury, M. Kim, C. M. Elvin, A. J. Hill, B. Thierry and K. Vasilev, Biomaterials  31: 4434-4446 (2010).5. M. Shaikh, G. Kichenadasse,  N.R.Choudhury, R.Butler, S.Garg, J. Controlled Release,  172:105-117 (2013).6. F. Ahmed, N.Roy Choudhury, N. K. Dutta, A. Zannettino, R. Knott Biomacromolecules  , 14(11):3850�3860 (2013).7.  J. Whittaker, R. Balu, N. Roy Choudhury, N. Dutta, Polymer International,  In Press 2013.

Multi stimuli reseponsive protein polymer and its nanobio conjugates

248th National Meeting (2014)

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