Powerful analytical tools can be produced by combining the simple components of yeast, hydrogel (from seaweed) and paper.   Saccharomyces cerevisciae were used to construct the first example of a yeast whole-cell, paper-based biosensor device. This biologically-based paper analytical device or �BioPAD� can qualitatively discriminate the presence/absence of doxycycline over a range of 30 � 10,000 �g/mL. Using a BioPAD, a doxycycline dosage form (tablet) commonly used for malaria prophylaxis, was confirmed to contain the antibiotic with 92% and 95% success, evaluated by eye and computer-assisted image analysis respectively, with no false positives by either method. Fabrication methods used in developing the BioPAD could be applied to other yeast biosensors to produce a variety of simple, analytical tools able to address a range of problems both in and outside of the laboratory setting.

Paper-based beast Biosensors for pharmaceutical screening

248th National Meeting (2014)

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