A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was modified and validated for the determination of residues of a systemic nematicide 'N', and two sulfonic acid metabolites, in green and cured tobacco leaves. The limits of quantitation and detection were 0.01 �g/g and 0.0033 �g/g, respectively, for all analytes. The method was modified to improve recoveries of N in green and cured leaves, with the addition of a second extraction. Additionally, for cured tobacco leaves, a pre-extraction hydrolysis step was employed. Matrix effects in the tobacco extracts were mitigated with the optimization of an SPE cleanup and the use of matrix-matched external standards. Our results show that this sensitive and relatively simple modified analytical method is suitable for the determination of trace residues of the subject analytes in green and cured tobacco leaves. The techniques described for tobacco leaves will be applied to other difficult-to-analyze dry and/or complex plant matrixes.

Determination of a systemic nematicide and acid metabolite residues in green and cured leaf tobacco: Minimizing quantitative effects from complex matrixes

248th National Meeting (2014)

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