Recent developments in stem-cell related regenerative medicine have raised requirements for an accurate control of cell�material interaction at the nanometer scale. Surface nanostructures were notably shown to be of a great interest to trigger cell functions. Among the relevant 3D nanostructures, nanopillars showed the unique capability to penetrate into cell interior which can be advantageously used to perform local drug delivery.Here we report on the development of nanopillars1,2 functionalized with redox-responsive copolymer layers leading to a local and temporally controlled administration of drug into cells (figure 1). Thiolactone-based copolymers3,4,5 releasing free thiol groups in presence of amine derivatives were successfully grafted onto gold nanopillars and flat gold surfaces used as controls. The resulting redox-responsive layers were subsequently modified with a fluorescent thiol derivative through a cleavable disulfide link. The microstructure, chemical composition, stability and redox-responsive properties of copolymer layers were systematically investigated as a function of their content in thiolactone units. Moreover, the ability of the modified surfaces to release a labeled substance under redox stimulation was demonstrated in physiological conditions. Finally, first tests were performed in presence of cells. Figure 1. <img src='https://acs-archive-assets2.s3.amazonaws.com/se_sw_rm/uploads/64836_dmg.jpg' alt='' /> 1Leprince, L. et al. J. Mater. Sci. Mater. Med. 2010 , 21, 925.2Lis, D. et al. Adv. Optical Mater. 2013 , 1, 244.3Reinicke, S. et al. ACS Macro Lett. 2013, 2, 539.4Espeel, P. et al. J. Am. Chem. Soc. 2011 , 133, 1678.5Espeel, P. et al. Polym. Chem. 2012 , 3, 1007.

Bioactive and stimuli-responsive nanopillars to spatially and temporally stimulate cells at the sub-cellular level

248th National Meeting (2014)

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