We have developed methods to create a biomimetic artificial membrane, through contact of water droplets in an oil solution containing lipids such as monoglycerides. Using optical microscopy, we have measured water transport between droplets, as water moves from one droplet to another due to concentration difference. This was assessed as a function of lipid content, structure, and additives, such as cholesterol, which is an essential component of human cells.  Our results show that cholesterol can increase the activation energy of water permeability several-fold, depending upon the structure of the monoglyceride lipid that makes up the bilayer, thus shedding light on how this singular sterol is vital to control of water movement.

Water permeability studies using a droplet interface bilayer: Effect of monoglyceride structure and cholesterol content

248th National Meeting (2014)

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