SERS based assays offer a number of potential advantages relative to conventional diagnostic platforms, including higher sensitivity and greater multiplexing capacity. Herein we describe our effort to develop a simple, rapid homogeneous SERS immunoassay based on analyte-mediated aggregation of gold nanoparticles. Gold nanoparticles are modified with a Raman reporter molecule and a molecular recognition element, e.g., antibody, to form an extrinsic Raman label (ERL). As well-dispersed particles, ERLs do not provide a hot spot and thus, do not produce a detectable SERS signal. In the presence of the antigen, the ERLs aggregate around the antigen thereby forming hot spots to facilitate SERS detection. Two model systems, influenza A virus and mouse IgG, are used to optimize reproducible formation of aggregates and establish proof-of-principle for the assay. Additionally, the size of the antibody used to modify the nanoparticles is implicated as the limiting factor in assay sensitivity.

SERS immunoassay based on analyte-mediated aggregation of gold nanoparticles

248th National Meeting (2014)

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