Bioorthogonal chemistry has enabled the visualization and study of biomolecules not directly encoded by the genome. Fluorogenic probes activated by a bioorthogonal reaction can offer significant advantages in imaging studies where it is challenging to wash away unreacted probe, such as real-time imaging of dynamic processes in cells or visualization in live organisms.  We present a platform for the computation-aided design of fluorogenic azide probes activated by the [3+2] cycloaddition reaction with alkynes. These probes feature emission wavelengths from the green to the near-infrared and are well-suited for the detection of alkynes in biological systems.

Development of fluorogenic azide probes for biological imaging

248th National Meeting (2014)

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