The use of artificial materials in medical applications requires a detailed understanding and control of the adhesion of cells to synthetic surfaces. Three main factors influence the interaction of cells and cell organelles with surfaces: topography, surface chemistry and mechanical properties of the substrate, as they all play a role in the interaction between surface, extracellular matrix and cell membrane. An interesting case of the behavior of cells on surfaces is the behavior of osteosarcoma cells (SaOs-2) on microstructured substrates. These bone cancer cells exhibit a strong deformation of the cell nucleus when brought into contact with micro-structured surfaces1.In our contribution we systematicallystudy the influence of the surface chemistry and shape of specifically designed micro-structured, polymer-coated surfaces on the adhesion and morphology of cells and their nuclei, which had been seeded onto these substrates. (Figure 1A-D). We generate large arrays of micropillared surfaces with variation of the size, height and distance of the pillars. In addition, we design the surfaces such that either the whole surface or only the top of the pillars or only the space between the pillars are made cell repellent and compare to situations where the whole microstructure is made cell adhesive.REFERENCES1. Davidson, P.M.; Ozcelik, H., Hasirci, V.; Reiter, G.; Anselme, K. Microstructured surfaces cause severe but non-detrimental deformation of the cell nucleus. Adv. Mater. 2009  , 21, 3586-3590

On the influence of surface chemistry and 3D microstructures on the shape of the cell nucleus

248th National Meeting (2014)

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