The interaction between biofilm forming bacteria and human tissue cells on plasma-mediated surface modifications, both separately and in co-culture, was studied. Specifically, this investigation focused on the adhesion and colonization of Staphylococcus aureus and human fetal osteoblasts on constructed intelligent surfaces. It is reported that variation in tetrazole polymer brush density in conjunction with polymer chain length, which are primarily based on plasma treatment and grafting conditions, demonstrate differential antibacterial and anti-biofilm activities. Further studies revealed under optimal experimental conditions, the antibacterial activity of grafted surfaces had two distinct mechanisms of action: 1) reducing bacterial attachment; and 2) eradicating adherent bacteria upon contact. The excellent antibacterial activity, along with biocompatibility of these sophisticated surfaces, were initially investigated through stationary cultures and subsequently confirmed through a co-culture, which mimicked in vivo conditions of an implanted device. The information gathered from this investigation suggests negatively charged brushes, achieved under appropriate graft polymerization conditions, may be employed to prevent biofouling on both implants and medical devices.

Surface modifications for the prevention of biofouling on implants and medical devices

248th National Meeting (2014)

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