Pesticide residues in ecosystems are serious environmental pollutants. Their toxic impact is linked to the quality of farm crops and food safety. In this study, genes coding for glucosyltransferases (GTs) were screened from isoproturon(IPU)-exposed wheat crops. The residual isoproturon and its metabolic products from plant tissues were structurally characterized using UPLC-TOF-MS/MS. Our analysis showed that isoproturon metabolic products can be glycosylated. Five glycosylation products were identified in wheat tissue. The GTs-responsive glycosylation of isoproturon has been found to be positively regulated by salicylic acid (SA), with increased amounts of isoproturon-glycosylated products detected in the presence of SA in SA-treated experiments. Multiple supporting data provide evidence that application of SA not only intensified the decay process of IPU by producing more glycosylated products, but also attenuated the toxic response of wheat plants to IPU, via reprogramming some genes responsible for toxicant detoxification and altering total GT activities and physiological response to IPU.

Effect of glucosyltransferases on degradation and detoxification of isoproturon residues in wheat in the presence of salicylic acid

248th National Meeting (2014)

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