Scab in one of the most common disease in cucumber, yet an appropriate in vivo micro-screening method (VMM) is currently not in place. In this paper, a VMM for agrochemicals is established, with the selection of etiolated cucumber seedlings. Through spraying agrochemicals (less than 2 mg a.i.) and then inoculating Cladosporium Cucumerinum, the pathogenetic cotyledon is observed in reddish brown after 3 days. This method significantly simplifies and improves the distinction of symptom, with 85% reduction in experimental space comparing to pot experiment in greenhouse. The proposed model is calibrated with 103 compounds and conventional screening in vivo, which achieves the same result. A novel methodology of utilizing ultraviolet spectrophotometry to evaluate cucumber scab is developed, which effectively minimizes the observation errors and increases the accuracy in disease classifications. The result shows a high correlation between the severity ratings of disease and the value of absorbance in 415 nm.

Introducing an in vivo microscreening model to agrochemicals for cucumber scab and quantitative evaluation technology

248th National Meeting (2014)

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