Abstract Controlled assembly of nanoparticles with high precision and high levels of integration has great applications in electronics and optics industry. Bio-nanoparticle namely tobacco mosaic virus (TMV) has a high aspect ratio that can be engineered to facilitate self-assembling on various substrates and also serve as a template for synthesis of nano-structured materials. TMV is a rod-like plant virus consisting of 2130 identical protein subunits arranged helically around a single stranded RNA,where tyrosine residues present on the exterior surface of 300nm long TMV particle was modified using click chemistry for efficient attachment of fluorescent beads through biotin-streptavidin complex. These fluorescent TMV were patterned within digital versatile disc (DVD) and Blu-Ray grooves thereby produced a linearly assembled TMV substrate. Furthermore, super high resolution of these patterned nanoparticles was performed using our reported Microlens based optical microscope technique, which has the ability to spatially resolve hard/soft nanoparticles separated by a distance of 100nm under a visible light source. UV-Visible and fluorescence spectroscopy, mass spectrometry, and dynamic light scattering were used to characterize the attachment of fluorophores on TMV, whereas fluorescence and scanning electron microscope were used for imaging the assembled fluorescent-TMV in Blu-Ray and DVD grooves. We demonstrate super-high resolution of fluorescence on these assembled TMVs utilizing Microlens based optical microscope. Keywords: Optical Microscope, Microlens, Tobacco mosaic virus, Fluorescence and DVD

Super-high resolution of control assembled fluorescent-TMV using microlens

249th National Meeting (2015)

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