Natural product extracts are known to be valuable for the discovery and production of potential pharmaceuticals. However, crude extracts present many challenges for biological testing, such as cytotoxicity, due to the extract containing a complex mixture of compounds. Using solid phase extraction, solvents of varying polarity produce unique fractions. These fractions allow natural products components to be separated and screened more efficiently for their potential use as an anticancer agent. The Molecular Target Laboratory (MTL) has fractionized over 22,000 natural products consisting of over 120,000 fractions which comprise the Molecular Target Laboratory Screening Library of Prefractionated Samples. Fractionalized products are tested in high-throughput screening applications for their respective biologically activity. Following screening of fractions it has been found that 10-30% of the samples which showed no indication of biological activity in their crude state exhibited measurable activity in one of their respective fractions following prefractionation. Prefractionation has proven a beneficial technique for effective evaluation of natural product extracts used in high-throughput anticancer screening applications.

Prefractionation of natural product extracts provides a value added resource for high-throughput anticancer screening applications

249th National Meeting (2015)

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