High temperature high-performance liquid chromatography (HT-HPLC) has great potential for uses in routine pharmaceutical analyses and can often be employed with little to no organic modifier, such as acetonitrile or methanol, in the mobile phase thereby lending to a green method of analysis. The use of HT-HPLC in the analyses of acetaminophen and caffeine has been investigated because of their presence in many different pharmaceutical formulations. Using a ZirChrom Diamondbond-C18 column and 100 % 0.7 M acetic acid at 3.00 mL/min, separations of acetaminophen and caffeine at temperatures between 60 oC and 110 oC were conducted. From the van’t Hoff analysis of the chromatographic data, the ∆H and ∆S for the mass transfer of these solutes between the mobile and stationary phases were evaluated. An analytical method was developed and evaluated for acetaminophen in a children’s liquid analgesic preparation by HT-HPLC using a ZirChrom Diamondbond-C18 column, 100 % 0.7 M acetic acid at 3.00 mL/min and temperature of 100 oC. Comparisons were made with results from conventional HPLC at ambient temperature using a BAKERBOND Octadecyl (C18) column with 10% methanol/90% 0.7 M acetic acid at 2.00 mL/min.

Separations of acetaminophen and caffeine by high temperature high-performance liquid chromatography

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.