The overall goal of this research project focuses on developing biosensors that are responsible for the detection of clinically relevant biomarkers. Sensors in general consist of target detection and signaling components. Once a target molecule is detected, it must then signal the binding event via a transducer. We are working towards building an accurate and efficient biosensor for relevant small molecules such as tryptophan, ATP and serotonin. Aptamer probes are used for detecting specific targets in combination with indirect signaling from a reactivated Glucose oxidase (GOx). The signaling mechanism of this experiment is to interpret the signal of flavin adenine dinucleotide (FAD) based reactivation of apo-GOx (enzyme without co-factor), which is released upon target binding. A direct signaling method is to detect FAD or FMN that is released via square wave stripping voltammetry or fluorescence spectroscopy. The current studies compare the different methods of detection of the released co-factors and aim to establish limits of detection for each method.

Direct and indirect detection of FMN and FAD released upon target binding

249th National Meeting (2015)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.