In many large genetic studies, the amount of available DNA can be one of the criteria for selecting samples for the study. Compared to those genomic DNA methods using organic solvents or commercial kits, the method based on MNPs is time-saving and cost-effective without the laborious centrifugation or precipitation steps, and has the great potential for automated DNA extraction. After the preparation of carboxyl-modified superparamagnetic nanoparticles, the yield and purity of genomic DNA based on MNPs are very better. In this paper, we compared and verify of genomic DNA extraction from different sources <i>serum</i> and whole blood based on magnetic nanoparticles. These genomic DNA extracted by using the MNPs method can all be available for the next molecule steps.

Extraction and verification of hepatitis B virus DNA from whole blood based on magnetic nanoparticles and whole genome amplification

249th National Meeting (2015)

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