One-pot multienzyme (OPME) systems containing glycosyltransferases and sugar nucleotide biosynthetic enzymes have seen great applications for synthesizing complex glycans and glycoconjugates including those containing naturally occurring and non-natural carbohydrate modifications. Identifying enzymes and mutants with good solubility and stability, high expression level in simple <i>Escherichia coli</i> expression systems, high activity, and promiscuous substrate specificity is the key to the high efficiency of the OPME chemoenzymatic synthesis. Our group's and others' efforts on this aspect, challenges, and future directions will be presented and discussed.

Carbohydrate biosynthetic enzymes for one-pot multienzyme (OPME) chemoenzymatic synthesis of glycans and glycoconjugates

249th National Meeting (2015)

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