We report on a massive coding of dissociated elements (MiCODE) technology to produce encoded freestanding microarrays that draw from certain advantages of conventional DNA microarrays, 3D photolithography as well as multiplex microspheres. The MiCODE microarray represents two significant advances. The first is the use of a new photolithography material to fabricate 3D structures of microarrays that could be released into biocompatible buffers, permitting higher flexibility in detection strategies and faster reaction kinetics in solution compared to conventional microarray technologies. Before, microarrays have been always in 2D structure and are fixed on solid substrates. Our MiCODE microarray is conceptually novel. The second is the versatility of encoding the suspended microarrays that are carried by micropallets. We demonstrate the printing of DNA arrays as well as various codes on each of the micropallets. They could also be encoded by UV photobleaching, a novel approach to generate high-resolution patterns on the micropallets. The encoded micropallets can be produced in a throughput of thousands to millions per fabrication batch. We envision the suspended MiCODE microarrays can find broad applications in the analysis of genetic and signaling information, drug screening, medical diagnostics and combinatorial chemistry, as well as the labeling of consumer goods or as security labels to prevent counterfeiting.

High-throughput production of multifunctional suspension microarrays by massive coding of dissociated elements

251st National Meeting (2016)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.