Highthroughput magnetic biocolloid technology is developed to screen drugs, chemicals, environmental pollutants and their reactive metabolites that have the potential to covalently bind to DNA fragments and possibly cause mutations. P53 gene fragments are specifically used in this study. P53 is tumor supressor gene and mutations with P53 gene are known in more than 50 % cancer. Most mutations with P53 gene extend from exon 5-8. Highthroughput magnetic biocolloid technology is coupled with LC-MS/MS to determine the codon specific chemical-nucleobase adduction and this information is translated to organ specific cancer based on databases available. Chemicals under study include Benzo[a]pyrene, Alfatoxin B1 and 2-Acetylamino fluorine and are known to be associated with lung, liver and bladder cancer. Exon fragments under consideration are codon 242 to 253 exon 7, codon 272 to 281 exon 8, codon 152 to 161 exon 5 & codon 172 to 181 exon 5.<br/>

Determination of tissue specific cancer of chemical/metabolites based on sequence specific DNA damage across the exons of P53 gene fragments using LC-MS/MS and Magnetic Bio-colloid technology

251st National Meeting (2016)

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