Current methods for the long-term preservation of mammalian cells, such as freezing and dehydration, are damaging to cells and often lead to a reduction in cell viability. Therefore, new conditions for the long-term storage of mammalian cells are needed to address the growing need for live cells in both research and clinical settings. The disaccharide trehalose is a naturally occurring sugar that is synthesized by a variety of organisms, including bacteria, yeast, fungi, and insects, when they are subjected to freezing or drying conditions. The exact mechanisms by which trehalose helps to protect these organisms from damage during these stressful conditions remains unknown. In order to be effective for cellular preservation, trehalose must be delivered to the intracellular environment; however, mammalian cells do not naturally produce trehalose. Furthermore, the hydrophilic nature of this disaccharide prevents it from freely entering into cells. To overcome this obstacle, we have synthesized a panel of novel trehalose analogs that are able to enter into mammalian cells.  We have quantified the intracellular levels of trehalose in Jurkat cells that have been incubated with these different trehalose analogs and are currently evaluating the protective effects of these analogs. Results from these studies will eventually lead to a more effective method for preserving live mammalian cells.

Synthesis and evaluation of trehalose analogs for the protection of mammalian cells

251st National Meeting (2016)

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