The development of high affinity protein-binding particles and materials has been an intensively studied area over the past two decades in biopharmaceutical industry for protein purification, and also in materials science for regenerative medicine and drug delivery. While the screening and identification of a ligand that can specifically interact with a protein of interest presents a key step towards the success of this strategy, further optimization of the binding affinity and the presentation of the identified ligand on a solid substrate are equally essential to realize the desired function. In this context, we report our recent progress in the development of self-assembling nanofibers that can specially bind to monoclonal antibodies (mAbs) and Fc-fusion proteins. The high binding affinity between the self-assembled peptide nanofibers and antibody were confirmed using isothermal titration calorimetry. The key design parameters underpinning the high binding affinity of these rationally-crafted synthetic materials will be analyzed and discussed.

Biomimetic approach to engineering high affinity protein-binding supramolecular nanofibers

253rd National Meeting (2017)

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